mouse anti chicken cd8 apc cd8α  (SouthernBiotech)

Journal: Microbiology Spectrum

Article Title: Amino acid insertion in the Meq protein of Marek’s disease virus, an avian oncogenic herpesvirus, accelerates tumorigenesis

doi: 10.1128/spectrum.03368-24

Figure Lengend Snippet: Gating strategy for the analysis of the dynamics of T-cell subsets involved in immune response in chickens infected with rMDVs. A representative gating strategy is shown for analyzing the dynamics of CD8α + γδ T cells, CD4 + γδ T cells, and CD8αα + T cells. Dead cells were excluded using Fixable Viability Dye eFluor780.

Article Snippet: The cells were stained with mouse anti-chicken CD3ε mAbs conjugated with PerCP-Cyanine5.5 dye, mouse anti-chicken CD4 mAbs conjugated with PE-Cy7, mouse anti-chicken CD8β mAbs conjugated with FITC, mouse anti-chicken CD4 mAbs conjugated with PE, and mouse anti-chicken CD8α mAbs conjugated with APC (Southern Biotech) for 30 min at 4°C in the dark.

Techniques: Infection

Journal: Microbiology Spectrum

Article Title: Amino acid insertion in the Meq protein of Marek’s disease virus, an avian oncogenic herpesvirus, accelerates tumorigenesis

doi: 10.1128/spectrum.03368-24

Figure Lengend Snippet: Dynamics of T-cell subsets involved in immune response in chickens infected with rMDVs. The dynamics of CD8α + γδ T cells and CD8αα + T cells from spleens of chickens infected with vRB-1B-Meq or vRB-1B-L-Meq at each time point during the experimental period (7–49 dpi: control group: n = 4, vRB-1B_Meq-infected group: n = 4, vRB-1B_L-Meq-infected group: n = 4, 56 dpi: control group: n = 4, vRB-1B_Meq-infected group: n = 7). ( A ) The percentages of CD8α + T cells in the γδ T cell population from spleens, ( B ) the percentage of CD8αα + cells in CD8α + T cell population from spleens, and ( C ) the percentage of CD4 + CD8α - T cells in CD4 + T cell population from spleens were analyzed. Error bars indicate standard deviations. * P < 0.05, Kruskal–Wallis test (7–35 dpi), Mann–Whitney U test (49–56 dpi).

Article Snippet: The cells were stained with mouse anti-chicken CD3ε mAbs conjugated with PerCP-Cyanine5.5 dye, mouse anti-chicken CD4 mAbs conjugated with PE-Cy7, mouse anti-chicken CD8β mAbs conjugated with FITC, mouse anti-chicken CD4 mAbs conjugated with PE, and mouse anti-chicken CD8α mAbs conjugated with APC (Southern Biotech) for 30 min at 4°C in the dark.

Techniques: Infection, Control, MANN-WHITNEY

Journal: Veterinary research

Article Title: Revealing novel and conservative CD8 + T-cell epitopes with MHC B2 restriction on ALV-J.

doi: 10.1186/s13567-024-01426-3

Figure Lengend Snippet: Figure 1 Detection of ALV-J shedding, ALV-J viremia, ALV-J antibody and T lymphocyte percentage of B2 haplotype chickens after ALV-J infection. Six chickens were randomly selected for sampling every 7 days post-infection (dpi). A ALV-J shedding was monitored via detecting the p27 expression levels in cloacal swabs. S/P value below 0.2 indicated negative ALV-J shedding. B ALV-J viremia was monitored via virus isolation. An S/P value above 0.2 indicated positive ALV-J viremia. C The ALV-J antibody level in the serum was monitored using the commercial ALV-J antibody test kit. An S/P value above 0.6 was considered ALV-J antibody positive. Five days before infection (dbi) and each week after infection, PBMCs derived from five chickens of infected and control groups were isolated to detect the T lymphocyte percentage, including the percentage of the CD4+CD3+T cell (D), the CD8α+CD3+T cell (E), and the CD3+CD4+CD8α+T cell (F). Each sample collected 1 × 105 cells for flow cytometric analysis. The one-way test was used for statistical comparison among A, B and C. And the unpaired Student t test was used for statistical comparison among D, E and F. * P < 0.05, ** P < 0.01.

Article Snippet: APC-conjugated mouse anti-chicken CD3+, PE-conjugated mouse anti-chicken CD8α+, FITC-conjugated mouse anti-chicken CD4+, (SouthernBiotech, Birmingham, USA) were used in this study.

Techniques: Infection, Sampling, Expressing, Virus, Isolation, Derivative Assay, Control, Comparison

Journal: Veterinary research

Article Title: Revealing novel and conservative CD8 + T-cell epitopes with MHC B2 restriction on ALV-J.

doi: 10.1186/s13567-024-01426-3

Figure Lengend Snippet: Figure 1 Detection of ALV-J shedding, ALV-J viremia, ALV-J antibody and T lymphocyte percentage of B2 haplotype chickens after ALV-J infection. Six chickens were randomly selected for sampling every 7 days post-infection (dpi). A ALV-J shedding was monitored via detecting the p27 expression levels in cloacal swabs. S/P value below 0.2 indicated negative ALV-J shedding. B ALV-J viremia was monitored via virus isolation. An S/P value above 0.2 indicated positive ALV-J viremia. C The ALV-J antibody level in the serum was monitored using the commercial ALV-J antibody test kit. An S/P value above 0.6 was considered ALV-J antibody positive. Five days before infection (dbi) and each week after infection, PBMCs derived from five chickens of infected and control groups were isolated to detect the T lymphocyte percentage, including the percentage of the CD4+CD3+T cell (D), the CD8α+CD3+T cell (E), and the CD3+CD4+CD8α+T cell (F). Each sample collected 1 × 105 cells for flow cytometric analysis. The one-way test was used for statistical comparison among A, B and C. And the unpaired Student t test was used for statistical comparison among D, E and F. * P < 0.05, ** P < 0.01.

Article Snippet: PBMCs (1 × 106) were incubated with mouse anti-chicken CD3 mAb (SouthernBiotech, Birmingham, USA), mouse anti-chicken CD4 mAb (SouthernBiotech, Birmingham, USA) and mouse anti-chicken CD8α mAb (SouthernBiotech, Birmingham, USA), respectively, at 4 °C for 30 min in the dark.

Techniques: Infection, Sampling, Expressing, Virus, Isolation, Derivative Assay, Control, Comparison